Muldoon LL, Pagel MA, Kroll RA, et al. A Physiological Barrier Distal to the Anatomic Blood-Brain Barrier in a Model of Transvascular Delivery. AJNR Am J Neuroradiol 1999;20:217–22
We investigated the delivery of ferumoxide (Feridex) iron oxide nanoparticles across the BBB in rats. The original goal was to use the MR contrast agent as a marker of viral-sized particle delivery to the brain. We administered Feridex intra-arterially (IA) in conjunction with hypertonic mannitol, a technique to safely and transiently open the BBB. MRI showed increased signal throughout the disrupted cerebral hemisphere, indicating global delivery of nanoparticles throughout the brain. In contrast, electron microscopy showed that the iron oxide nanoparticles were located at the basement membrane of cerebrovascular endothelial cells (Figure 1). Thus, the nanoparticles had crossed the BBB but had not actually entered brain parenchyma. This is important for clinical practice regarding the use of nanoparticles, such as encapsulated drugs, as delivery vehicles. Our results show that the presence of these agents in the brain does not necessarily indicate their accessibility to the target cells, either neurons or tumor cells.
This AJNR paper was the first of many preclinical studies in my lab investigating multiple aspects of imaging with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles. In 2005, we assessed delivery and toxicity of Feridex and alternative USPIO nanoparticle agents, including ferumoxtran-10 (Combidex) and ferumoxytol, in rat brain.1 In this study, Feridex was stuck at the basement membrane for at least a month after transvascular delivery, while ferumoxytol was dispersed through brain tissue and could no longer be detected 1 week later (Figure 2). It is important for the use of ferumoxytol in neuroradiology that delivery directly to brain tissue was safe and gave relatively transient (1 week) signal changes.